Dirofilaria immitis, the causative agent of canine heartworm disease, and West Nile virus (WNV) are both mosquito-borne pathogens. Mosquito surveillance and vector determination are crucial in understanding the transmission of these pathogens which helps implement methods of control to prevent and predict outbreaks. In this study I assessed the ability of the Anopheles quadrimaculatus complex to transmit D. immitis and investigated the influence climate and land use and land changes (LULC) have on WNV transmission in Culex quinquefasciatus mosquitoes, the primary vector in the Southeastern United States. Standard PCR and an L3-specific RT-PCR protocol were used to assess D. immitis infection rates in An. quadrimaculatus mosquito samples. To determine the influence of climate and LULC on WNV from 2012-2021, data were collected from Weather Underground and the VSU WNV Mosquito Surveillance lab. Results from this study suggest that the An. quadrimaculatus complex is a potential vector of D. immitis and that urbanization and temperature influence WNV transmission by mosquitoes in Lowndes Co., GA.