Statistical Analysis of Sequence Characteristics of Single Transmembrane Cluster of Differentiation Proteins: A Study of Functional Relevance
Loading...
Authors
Patterson, Ashlie Kiel
Issue Date
2012-01-26
Type
Dissertation
Language
en_US
Keywords
Transmembrane Cluster , Protiens , Biology , proteomics , Cluster of Differentiation , diagnostic techniques , immunological , white blood cell
Alternative Title
Abstract
Cluster of Differentiation (CD) proteins are human white blood cell markers
involved in immune reactions. They are used as targets for immunological studies,
diagnostic techniques of disease states as well as utilized in therapeutic applications for
cancers and other maladies of the body. Analysis of sequence characteristics of CD
proteins has enabled the identification of functional trends that may be useful in
understanding their roles within the immune system. Through statistical analysis,
Principal components analysis (PCA) and K-means clustering, 36 Type II CD proteins
were analyzed by one hundred twenty-four biochemical and biophysical properties
derived from amino acid sequences alone. The analysis revealed these 36 CD proteins
can effectively be grouped into two distinct known functional groups. In addition,
possible unknown functional properties of CD71 have been identified specifically. This
research shows the potential for a quantitative approach in proteomics to unlock
predictive capabilities. It is believed that upon the basis of this analytical technique,
functional properties may be predicted of currently undiscovered CD proteins as well as
known, poorly characterized CD proteins.