An Economical Synthesis Route in the Ocean for the Marine Natural Product, Bryostatin
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Authors
Falkenhausen, Thomas
Cowan, Matt
Tidwell, Michael
Issue Date
2020
Type
Presentation
Language
en_US
Keywords
Alternative Title
Abstract
Bryostatin is a marine natural product found in minute amounts in nature. Cost 17 million USD/gram, this molecule targets latent HIV reservoirs, has potential for slowing the progression of Alzheimer's, and demonstrates strong anti-cancer properties. We have developed an economical synthesis of bryostatin through the creation of a bioreactor that cultivates a symbiotic bacterium which lives on the bryosome B. Nerintina. This bioreactor is comprised of inexpensive organic materials providing essential nutrients which promotes the growth of the bacterium. Our bioreactor was deployed off the Gulf Coast for a short period to attract our symbiotic bacterium. This bioreactor was returned to the lab where it was monitored weekly for 2 months with sample extraction every other day. Each sample was labeled and stored in cold storage to prevent the degradation of bryostatin. After our collection period, we ran each sample through a UV Spectrometer to determine the possible presence of Bryostatin. Promising samples were, then, ran through a liquid chromatography mass spectrometer to determine the different types of Bryostatin present.