Over the course of several months, water samples were collected and measured along the Suwannee River Basin. The recorded rivers along the basin were Little River, Withlacoochee River, and Mud Swamp. These locations were distributed to monitor incoming flow from the head to the base of the basin near the Georgia Florida border line. Physical and chemical measurements such as PH levels, temperature, total dissolved solids, and total dissolved oxygen were reported. Chemical elements suspended in the water were also identified using the Atomic Absorption Spectrometry Machine. These methods help provide a better understanding of our water quality as well as the impact on surrounding ecosystems along our local river basin.