dc.contributor.author	Newsome, Jennifer
dc.contributor.author	Moore, Ginger
dc.contributor.author	Ard, Melissa
dc.contributor.author	Sucar, Sofia
dc.date.accessioned	2011-05-25T18:30:20Z
dc.date.available	2011-05-25T18:30:20Z
dc.date.issued	2011-05-25
dc.identifier.uri	http://hdl.handle.net/10428/1017
dc.description.abstract	The mangrove killifish is unique among vertebrates due to its self-fertilizing mode of reproduction. Here, we describe a three generation forward genetic screen to identify zygotic mutants which show phenotypes that disrupt early embryonic development. We treated parental fish (P; N = 34) with increasing doses of N-Ethyl-N-Nitrosurea (ENU) which induces point mutations in the germ-line in other model organisms. These P fish were self-crossed, producing 7,350 F1 progeny. 1,334 F1 fish were hatched and raised to maturity (18%). From these F1 fish, 284 were allowed to self-cross (284 genomes) and their F2 offspring were observed during embryogenesis for mutant phenotypes disrupting early development (zygotic mutants). We are currently observing these zygotic mutants into the F3 generation. Documented here are the types and frequencies of these zygotic mutants in our ongoing screen.	en_US
dc.language.iso	en_US	en_US
dc.subject	Mangrove Killfish	en_US
dc.subject	Zygotic Mutants	en_US
dc.subject	Gonad Development	en_US
dc.subject	Genetics	en_US
dc.title	Zygotic Mutants of the Mangrove Killifish, Kryptolebias marmoratus	en_US
dc.type	Learning Object	en_US
dc.type	Presentation	en_US
dc.type	Other	en_US