dc.contributor.author	Ward, Danielle Marie
dc.coverage.spatial	Louise, Lake. Stephens, Georgia, United States	en_US
dc.coverage.temporal	2018-2020	en_US
dc.date.accessioned	2020-07-15T16:33:39Z
dc.date.available	2020-07-15T16:33:39Z
dc.date.issued	2020-04
dc.identifier.other	C0C9D017-6A27-9A8A-4B9A-AE12FC825832	en_US
dc.identifier.uri	https://hdl.handle.net/10428/4268
dc.description.abstract	Lake Louise, a temperate lake in south-central Georgia, USA, is 6.5 meters deep at its deepest point and approximately 6 hectares in area. Despite being relatively shallow, the lake stratifies each year, typically stabilizing from mid-March until mid-November, with a thermocline located at a depth of 2.0 – 2.5 meters. During the summer, high tannin concentrations in the water also cause a sharp decrease in light levels with less than 0.1% of incident light reaching a depth of 2.5 m. However, spectrophotometric pigment analysis indicates that a distinct deep chlorophyll maximum (DCM) develops at an approximate depth of 3.0 m, well below the photic zone, and contains higher apparent chlorophyll b concentrations than the surface waters. Photosynthetic activity at this depth suggests organisms which are capable of compensating for extremely low-light. Preliminary data at 3.0 m indicate an abundance of filamentous, photosynthetic organisms that are visually consistent with cyanobacteria. Chlorophyll spectra from sampling events suggested bacteriochlorophyll d as the primary photosynthetic pigment in the DCM, while initial imaging of filters and sequencing of 16s RNA PCR amplicons further indicated the presence of eubacteria in the phyla Chlorobi and Chloroflexi. A Chloronema-type trichome has been identified through light micrographs that may be indicative of a novel morphospecies. Keywords: Chlorophyll; Phytoplankton; Chloronema; Thermal stratification;	en_US
dc.description.tableofcontents	Chapter I: INTRODUCTION 1 -- Chapter II: MATERIALS AND METHODS 5 -- Lake Louise 5 -- Environmental Monitoring 6 -- Biological Sample Collection 7 -- Pigment Analysis 7 -- Imaging Flow Cytometry and Light Microscopy 9 -- Isolation of Photosynthetic Organisms 10 -- Genetic Analysis 11 -- Cloning and Sequencing 14 -- Chapter III: RESULTS 16 -- Environmental Data 16 -- Chlorophyll Spectrum and HPLC Analysis 17 -- Microflora Morphology and Characteristics 20 -- Sequence Data 21 -- Chapter IV: DISCUSSION 23 -- REFERENCES 30 -- APPENDIX A: Approval of MUA for Recombinant DNA Experiments 39 -- APPENDIX B: Charts and Figures 43	en_US
dc.format.extent	1 electronic document, 63 pages	en_US
dc.format.mimetype	application/pdf	en_US
dc.language.iso	en_US	en_US
dc.rights	This dissertation is protected by the Copyright Laws of the United States (Public Law 94-553, revised in 1976). Consistent with fair use as defined in the Copyright Laws, brief quotations from this material are allowed with proper acknowledgement. Use of the materials for financial gain with the author's expressed written permissions is not allowed.	en_US
dc.subject	Academic theses	en_US
dc.subject	Chlorophyll	en_US
dc.subject	Georgia--Stephens County	en_US
dc.subject	Phytoplankton	en_US
dc.subject	Thermal analysis	en_US
dc.title	Investigation of the Deep Chlorophyll Maximum in Lake Louise, Georgia	en_US
dc.type	Thesis	en_US
dc.contributor.department	Department of Biology of the College of Science and Mathematics	en_US
dc.description.advisor	Nienow, James A.
dc.description.committee	Turco, Jenifer
dc.description.committee	Goddard, Russell H.
dc.description.degree	M.S.	en_US
dc.description.major	Biology	en_US