dc.contributor.author	Evans, Emily Erin
dc.coverage.spatial	Valdosta (Ga.)	en_US
dc.coverage.temporal	2019	en_US
dc.date.accessioned	2021-09-27T17:50:42Z
dc.date.available	2021-09-27T17:50:42Z
dc.date.issued	2021-07
dc.identifier.other	6EAE6CEB-2833-C7A8-4D16-D3EE869B731A	en_US
dc.identifier.uri	https://hdl.handle.net/10428/5014
dc.description.abstract	Mosquito populations are likely to develop genetic resistance to insecticides with prolonged and/or improper application. Regular assessments of insecticide resistance should be performed on exposed populations to optimize local integrated mosquito management strategies. Current strategies within Valdosta, Georgia, especially chemical insecticide application, may prove to be outdated and inefficient due to the absence of a designated resistance testing center. The current study aims to provide a baseline resistance report for the nuisance biter and potential disease vector, Aedes albopictus (Skuse) to the type I pyrethroid, permethrin. Although Ae. albopictus is primarily a nuisance biter in southern Georgia, this species has the physiological potential to be a vector of numerous arboviruses and other pathogens. Ae. albopictus colonies were established from eggs collected at 15 independent sites within and near Valdosta, GA between July and November of 2019. The majority of the sites were located in residential neighborhoods. Post-rearing, survivability upon permethrin exposure was tested using the Centers for Disease Control and Prevention (CDC) Bottle Bioassay Kit. This bioassay accounts for resistance by comparing a ‘diagnostic time’ (the maximum time required to kill 100% of susceptible mosquitoes of a particular species and insecticide dosage) to the actual time taken for experimental mosquitoes to die. The occurrence of permethrin resistance in Valdosta populations of Ae. albopictus was observed in this study. According to World Health Organization (WHO) standards, <90% mortality at the diagnostic time indicates resistance, and mosquitoes from all 15 collection sites exhibited mortality <82%. This level of resistance suggests that mosquito abatement methods currently used in Valdosta, Georgia should be modified to mitigate insecticide resistance. Population reduction of this potential vector species should then reduce the risk of rapid disease transmission should the respective pathogens be introduced into the area. Keywords: Permethrin, Pyrethroid, Insecticide Resistance, Asian Tiger Mosquito, Aedes albopictus, CDC Bottle Bioassay	en_US
dc.description.tableofcontents	ABSTRACT i -- DEDICATION ii -- CHAPTER I: INTRODUCTION 1 -- CHAPTER II: MATERIALS AND METHODS 9 -- Collection Sites 9 -- Oviposition Cups 10 -- Specimen Storage and Rearing 10 -- CDC Bottle Bioassay Resistance Testing 11 -- Statistical Analysis 13 -- CHAPTER III: RESULTS 14 -- CHAPTER IV: DISCUSSION 16 -- REFERENCES 22 -- APPENDIX A: TABLES 28 -- APPENDIX B: FIGURES 36	en_US
dc.format.extent	1 electronic document, 54 pages. 2416379 bytes.	en_US
dc.format.mimetype	application/pdf	en_US
dc.language.iso	en_US	en_US
dc.rights	This dissertation is protected by the Copyright Laws of the United States (Public Law 94-553, revised in 1976). Consistent with fair use as defined in the Copyright Laws, brief quotations from this material are allowed with proper acknowledgement. Use of the materials for financial gain with the author's expressed written permissions is not allowed.	en_US
dc.subject	Aedes albopictus	en_US
dc.subject	Biological assay	en_US
dc.subject	Dissertations, Academic--United States	en_US
dc.subject	Insecticide resistance	en_US
dc.subject	Mosquitoes	en_US
dc.subject	Pyrethroids	en_US
dc.title	Surveillance of Permethrin Resistance in Valdosta Populations of Aedes albopictus via the CDC Bottle Bioassay	en_US
dc.type	Thesis	en_US
dc.contributor.department	Department of Biology of the College of Science and Mathematics	en_US
dc.description.advisor	Blackmore, Mark S.
dc.description.committee	Chambers, Eric W.
dc.description.committee	Lockhart, J. Mitchell
dc.description.degree	M.S.	en_US
dc.description.major	Biology	en_US